Rockland/Akt phospho S473 Antibody/200-301-268/100 µg
商品编号：
200-301-268
品牌：
rockland-inc
市场价：
¥7180.00
美元价：
4308.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AktphosphoS473AntibodyProperties Description Anti-AKTpS473(MOUSE)MonoclonalAntibody-200-301-268 Host Mouse TargetSpecies Human KnownCrossReactivity human,mouse,rat,monkey Clonality Monoclonal17F6.B11IgG1kappa Application ELISA:1:20,000 IFMicroscopy:1:500-1:3,000 FlowCytometry:UserOptimized WesternBlot:1:500-1:3,000 Immunohistochemistry:20µg/mL OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG1 Label Unconjugated Concentration 1.0mg/mlbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName AKT1 RelevantLinks UniProtKB-P31749 NCBI-62241011 GeneID-207 |
AktphosphoS473AntibodyDescription
Background
PhosphoAKTantibodyissuitableforELISA,immunohistochemistry,immunoprecipitationandwesternblotting.AKTphospho473isacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKTpS473(MOUSE)MonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.
Synonyms
mouseanti-AKTpS473Antibody,phosphoAKT,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1
Immunogen
Anti-AKTpS473(MOUSE)MonoclonalAntibodywasproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoresiduessurroundingS473ofhumanAKT1protein,followedbyhybridomadevelopment.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
StoreAnti-AKTpS473(MOUSE)MonoclonalAntibodyat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
Expectabandapproximately56kDainsizecorrespondingtophosphorylatedAKTproteinbywesternblottingintheappropriatecelllysateorextract.Thisphospho-specificmonoclonalantibodyreactswithhumanandmouseAKTpS473andshowsminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistryuseformalin-fixedparaffin-embeddedsections.Nopre-treatmentofsampleisrequired.
Purity/Specifity
Anti-AKTpS473MonoclonalAntibodywaspurifiedfromconcentratedtissueculturesupernatebyProteinAchromatography.ThisphosphospecificmonoclonalantibodyisspecificforphosphorylatedhumanandmouseAKTproteinatS473.ABLASTanalysiswasusedtosuggestcross-reactivitywithAKTpS473fromhuman,mouse,ratandchimpanzeesourcesbasedon100%homologywiththeimmunizingsequence.Cross-reactivitywithAKTfromothersourceshasnotbeendetermined.Cross-reactivitywithAKT2andAKT3hasnotbeendetermined.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
品牌介绍
美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。
