Rockland/AKT1 APC抗体/200-326-I51/50µg
商品编号：
200-326-I51
品牌：
rockland-inc
市场价：
¥7180.00
美元价：
4308.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AKT1APCAntibodyProperties Description Anti-AKT1(MOUSE)AllophycocyaninConjugatedMonoclonalAntibody-200-326-I51 Host Mouse TargetSpecies Human KnownCrossReactivity Human,Mouse,Rat Clonality Monoclonal14E5.A2.B2.H9IgG2akappa Application ELISA:UserOptimized IFMicroscopy:Useroptimized FlowCytometry:1.0µg/mL WesternBlot:UserOptimized Immunohistochemistry:Useroptimized PhysicalState Lyophilized ShippingCondition WetIce Format IgG2a Label Allophycocyanine FPValue 1-2molesAllophycocyaninepermoleofIgG2a Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.5MSodiumChloride,pH7.2 ReconstitutionVolume 50µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative None StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName AKT1 RelevantLinks UniProtKB-P31749 NCBI-NP_001014431.1 GeneID-207 |
AKT1APCAntibodyDescription
Background
AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways.AKTExhibitstightcontrolovercellproliferationandcellviability.OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer.AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephosphorylated,AKTdissociatesfromthemembraneandphosphorylatestargetsinthecytoplasmandthecellnucleus.AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.Anti-AKT1(MOUSE)APCconjugatedMonoclonalAntibodyisidealforinvestigatorsinvolvedinCellSignaling,Cancer,Neuroscience,SignalTransductionresearch.
Synonyms
mouseanti-AKT1antibodyAPCconjugation,allophycocyaninconjugatedmouseanti-AKT-1antibody,AKT-1,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt1antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma
Immunogen
Anti-AKT1AntibodywasproducedinmicebyrepeatedimmunizationswithasyntheticpeptidecorrespondingtointernalresiduesofhumanAKT1proteinfollowedbymonoclonaldevelopment.
ImmunogenType
Peptide
StorageCondition
Storevialat4°Cpriortorestoration.Restorewithdeionizedwater(orequivalent).Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Forextendedstorage,aliquotcontentsandfreezeat-20°Corbelow.Avoidcyclesoffreezingandthawing.Storereagentinthedark.UsesuBDuedlightingduringhandlingandincubationofcellspriortoanalysis.
ApplicationNote
Anti-AKT1APCAntibodyissuitableforFlowCytometry,ELISA,immunohistochemistry,andwesternblotting.Expectabandapproximately56kDainsizecorrespondingtoAKT1proteinbywesternblottingintheappropriatecelllysateorextract.ThismonoclonalantibodyreactswithhumanAKT.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Forimmunohistochemistrywerecommendtheuseoffreshfrozentissues.Attemptsatstainingparaffin-embeddedformalinfixedtissueswerenegative.Nopre-treatmentofsampleisrequired.
Purity/Specifity
Anti-AKT1antibodyisdirectedagainsthumanAKT1.Theantibodydetectsbothunphosphorylatedandphosphorylatedformsoftheprotein.Anti-AKT1antibodywaspurifiedfromascitesbyProteinAchromatography.CrossreactivitywithAKT1fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows85%identitytomouseand92%identitywithrat,therefore,crossreactivityisexpected.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
品牌介绍
美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。
