Rockland/14-3-3磷酸化S58抗体/600-401-D02/100µL
商品编号：
600-401-D02
品牌：
rockland-inc
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
14-3-3phosphoS58AntibodyProperties Description Anti-14-3-3pS58(Rabbit)Antibody-600-401-D02 Host Rabbit TargetSpecies Rat KnownCrossReactivity rat,human Clonality Polyclonal Application PhysicalState Liquid ShippingCondition DryIce Format IgG Label Unconjugated Buffer 0.01MHEPES,0.15MSodiumChloride,pH7.5 StABIlizer 0.1mg/mlBovineSerumAlbumin(BSA)-IgGandProteasefree,50%(v/v)Glycerol GeneName YWHAB RelevantLinks UniProtKB-P35213 GeneID-56011 NCBI-NP_062250.1 |
14-3-3phosphoS58AntibodyDescription
Background
14-3-3pS58antibodydetects14-3-3proteinphosphorylatedatS58.14-3-3isamemberofafamilyofhighlyconservedproteinsthatappeartohavemultiplerolesincellsignaling.14-3-3isabundantlyexpressedinthebrainandhasbeendetectedinthecerebrospinalfluidofpatientswithdifferentneurologicaldisorders.14-3-3proteinsbindproteinligandsthataretypicallyphosphorylatedonserineorthreonineresiduesandregulatethefunctionsofthesebindingpartnersbyanumberofdifferentmechanisms.14-3-3affectadiversearrayofcellularprocessesincludingthecellcycleandtranscription,signaltransductionandintracellulartrafficking.Thesefunctionsarefacilitatedby,ifnotdependenton,itsdimericstructure.Recentworkhasdemonstratedthatthedimericstatusofthe14-3-3proteinisregulatedbysite-specificserinephosphorylation.Anti-14-3-3antibodyisidealforinvestigatorsinvolvedinCellSignaling,Neuroscience,SignalTransductionresearch.
Synonyms
14-3-3proteinbeta/alpha,PrepronervegrowthfactorRNH-1,ProteinkinaseCinhibitorprotein1
Immunogen
Anti-14-3-3pS58antibodywasproducedbyrepeatedimmunizationswithsyntheticphospho-peptidecorrespondingtoaminoacidresiduessurroundingSer58.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat-20°Cpriortoopening. Thisproductisstableat4°Casanundilutedliquid. Forextendedstorage,aliquotcontentsandfreezeat-20°Corbelow. Avoidcyclesoffreezingandthawing.Diluteonlypriortoimmediateuse.
ApplicationNote
Anti-14-3-3pS58antibodyissuitableforuseinELISA,WesternBlottingandIHC.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Expectabandofapproximately29kDainsizecorrespondingtothe14-3-3proteinsphosphorylatedatSer58intheappropriatecelllysateorextract.
Purity/Specifity
Anti-14-3-3pS58antibodyisdirectedagainstrat14-3-3proteinphosphorylatedatS58.Theantibodywaspreparedfrommonospecificantiserumbyimmunoaffinitychromatographyusingphosphopeptidecoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)againstnon-phosphopeptideandnon-specificpeptidetoremoveanyunwantedreactivities.Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerum.Thisantibodyisspecificforphosphorylated14-3-3.Minimalreactivityoccursagainstnon-phosphorylated14-3-3.Reactivityagainst14-3-3occursfromratandhumansources.Reactivityisexpectedagainstthefollowingspeciesbasedon100%sequencehomology:bovine,canine,chicken,mouse,non-humanprimates,sheep,Xenopusandzebrafish.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
品牌介绍
美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。
