Rockland/AHA1抗体/600-401-974/100µg
商品编号：
600-401-974
品牌：
rockland-inc
市场价：
¥7180.00
美元价：
4308.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AHA1AntibodyProperties Description Anti-AHA1(RABBIT)Antibody-600-401-974 Host Rabbit TargetSpecies Human KnownCrossReactivity human,chimpanzee Clonality Polyclonal Application ELISA:1:35,000-1:185,000 WesternBlot:1µg/mL Immunohistochemistry:UserOptimized OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName AHSA1 RelevantLinks UniProtKB-Q8BK64 NCBI-6912280 GeneID-10598 |
AHA1AntibodyDescription
Background
Thisantibodyisdesigned,produced,andvalidatedaspartofacollaborationbetweenRocklandandtheNationalCancerInstitute(NCI)andissuitableforCancer,ImmunologyandNuclearSignalingresearch.ActivatorofHsp90ATPase(AHA1)stimulatestheinherentATPasecycleofHsp90,whichisessentialforitschaperoneactivityinvivo. Theactivationand/orstabilityofmanyofthekeyregulatoryandsignalingproteinsoftheeukaryoticcelldependontheirinteractionwiththeHsp90molecularchaperone. Hsp90isassistedandregulatedbyco-chaperonesthatparticipateinanorderedseriesbothtoassistclient-proteinrecruitmentorreleaseandtomodulateprogressthroughtheATPasecoupledchaperonecycle. StructuralanalysisandmutagenesisshowthatbindingoftheN-terminaldomainofAHA1toHsp90promotesaconformationalswitchinthemiddle-segmentcatalyticloop(aa370–390)ofHsp90thatexposesthecatalyticArg380andenablesitsinteractionwithATPintheN-terminalnucleotide-bindingdomainofthechaperone. RecentstudiesshowthatAHA1modulatesHsp90-dependentstabilityofthefoldingofthecysticfibrosistransmembraneconductanceregulator(CFTR)intheendoplasmicreticulum(ER). Down-regulationofAHA1rescuesmisfoldingofCFTRincysticfibrosis.
Synonyms
rabbitanti-AHA1Antibody,Aha-1,Aha1,Ahsa1antibody,ActivatorofHsp90ATPase,Activatorof90kDaheatshockproteinATPasehomolog1antibody
Immunogen
ThisaffinitypurifiedantibodywaspreparedfromwholerabbitserumproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaninternalregionofhumanAHA1protein.
ImmunogenType
Peptide
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
ThisaffinitypurifiedantibodyhasbeentestedforuseinELISAandwesternblotting. Specificconditionsforreactivityshouldbeoptimizedbytheenduser. Expectabandapproximately38-40kDainsizecorrespondingtoAHA1proteinbywesternblottingintheappropriatecelllysateorextract.
Purity/Specifity
ThisaffinitypurifiedantibodyisdirectedagainsthumanAHA1protein. Theproductwasaffinitypurifiedfrommonospecificantiserumbyimmunoaffinitychromatography. ABLASTanalysiswasusedtosuggestcross-reactivitywithAHA1proteinfromhumanandchimpanzeebasedon100%homologywiththeimmunizingsequence. Reactivityagainsthomologuesfromothersourcesisnotknown.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
品牌介绍
美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。
