Rockland/AKT pS473抗体/600-401-268/100µg
商品编号：
600-401-268
品牌：
rockland-inc
市场价：
¥7180.00
美元价：
4308.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AKTpS473AntibodyProperties Description Anti-AKTpS473(RABBIT)Antibody-600-401-268 Host Rabbit TargetSpecies Human KnownCrossReactivity Human,Mouse,Rat Clonality Polyclonal Application ELISA:1:15,000-1:60,000 FlowCytometry:UserOptimized WesternBlot:1:200-1:1000 Immunohistochemistry:1:100-1:500 OtherDilution:UserOptimized PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName AKT1 RelevantLinks NCBI-62241011 UniProtKB-P31749 GeneID-207 |
AKTpS473AntibodyDescription
Background
AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationat Ser473andThr308. AKTisacytoplasmicproteinalsoknownasAKT1,ProteinKinaseB(PKB)andrac(relatedtoAandCkinases).AKTisakeyregulatorofmanysignaltransductionpathways. AKTExhibitstightcontrolovercellproliferationandcellviability. OverexpressionorinappropriateactivationofAKTisnotedinmanytypesofcancer. AKTmediatesmanyofthedownstreameventsofPI3-kinase(alipidkinaseactivatedbygrowthfactors,cytokinesandinsulin).PI3-kinaserecruitsAKTtothemembrane,whereitisactivatedbyPDK1phosphorylation.Oncephos-phorylated,AKTdissociatesfromthemembraneandphos-phorylatestargetsinthecytoplasmandthecellnucleus. AKThastwomainroles:(i)inhibitionofapoptosis;(ii)promotionofproliferation.
Synonyms
rabbitanti-AKTpS473Antibody,AKT1phosphoS473,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1
Immunogen
ThiswholerabbitserumwaspreparedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoaC-terminusregionnearaa460-480ofhuman,mouse,ratandchickenAKTproteinsconjugatedtoKLH.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat-20°Cpriortoopening.Aliquotcontentsandfreezeat-20°Corbelowforextendedstorage.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
ThisantibodyisphosphospecificforpS473andissuitableforwesternblotting,immunohistochemistry(formalin-fixedparaffin-embeddedsections),immunofluorescenceandELISA.Byimmunoblotasinglebandoftheexpectedapparentmolecularweightisobserved. Forimmunohistochemistrynopre-treatmentofsampleisrequired.
Purity/Specifity
Thisproductwaspreparedfrommonospecificantiserumbyimmunoaffinitychromatographyusingphosphopeptidecoupledtoagarosebeadsfollowedbysolidphaseadsorption(s)againstnon-phosphopeptideandnon-specificpeptidetoremoveanyunwantedreactivities.Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerum. ThisantibodyisspecificforphosphorylatedhumanAKTpS473. Minimalreactivityoccursagainstnon-phosphorylatedAKT. ReactivityagainstAKTfromotherspeciesmayoccurbuthasnotyetbeentested.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
品牌介绍
美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。
