Rockland/AKT pT308单克隆抗体生物素结合/200-306-269/50µg
商品编号：
200-306-269
品牌：
rockland-inc
市场价：
¥5380.00
美元价：
3228.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AKTpT308MonoclonalAntibodyBiotinConjugatedProperties Description Anti-AKTpT308(MOUSE)MonoclonalAntibodyBiotinConjugated-200-306-269 Host Mouse TargetSpecies Human KnownCrossReactivity human,mouse,rat,monkey Clonality Monoclonal18F3.H11IgG1kappa Application ELISA:1:20,000 FlowCytometry:UserOptimized WesternBlot:1:500-1:3,000 Immunohistochemistry:20µg/ml OtherDilution:UserOptimized PhysicalState Lyophilized ShippingCondition Ambient Format IgG1 Label Biotin FPValue 10-20molesBiotinpermoleofIgG1 Concentration 1.0mg/mLbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 ReconstitutionVolume 50µL ReconstitutionBuffer Restorewithdeionizedwater(orequivalent) Preservative 0.01%(w/v)SodiumAzide StABIlizer 10mg/mLBovineSerumAlbumin(BSA)-ImmunoglobulinandProteasefree GeneName AKT1 RelevantLinks UniProtKB-P31749 GeneID-207 NCBI-62241011 |
AKTpT308MonoclonalAntibodyBiotinConjugatedDescription
Background
Anti-AKTphosphoT308isidealforwesternblotting,ELISA,IHCandIP.PhosphoAKTpT308antibodyisspecificforAKTproteinphosphorylatedatT308.AKTisacomponentofthePI-3kinasepathwayandisactivatedbyphosphorylationatSer473andThr308.Anti-AKTpT308monoclonalantibodyisidealforinvestigatorsinvolvedinCancer,CellSignaling,Neuroscience,SignalTransductionresearch.
Synonyms
mouseanti-AKTpT308BiotinconjugatedAntibody,Biotinconjugatedmouseanti-AKTpT308Antibody,RAC-PK-alpha,ProteinkinaseB,PKB,C-AKT,RAC-alphaserine/threonine-proteinkinase,Proto-oncogenec-Akt,AKT1,AKT1,AKT-1,AktphosphoT308Antibody,Anti-AKTpT308MonoclonalAntibodyBiotinConjugated
Immunogen
Anti-AKTpT308monoclonalantibodywasproducedbyrepeatedimmunizationswithasyntheticpeptidecorrespondingtoresiduessurroundingT308ofhumanAKT1protein.
ImmunogenType
Peptide
PostTranslationalModification
Phosphorylation
StorageCondition
Storevialat4°Cpriortorestoration.Forextendedstoragealiquotcontentsandfreezeat-20°Corbelow.Avoidcyclesoffreezingandthawing.Centrifugeproductifnotcompletelyclearafterstandingatroomtemperature.Thisproductisstableforseveralweeksat4°Casanundilutedliquid.Diluteonlypriortoimmediateuse.
ApplicationNote
BiotinConjugatedAnti-AKTpT308issuitableforELISA,immunohistochemistry,immunoprecipitationandwesternblotting.Expectabandapproximately56kDainsizecorrespondingtophosphorylatedAKTproteinbywesternblottingintheappropriatecelllysateorextract.Thisphospho-specificmonoclonalantibodyreactswithhumanandmouseAKTpT308andshowsminimalreactivitybyELISAagainstthenon-phosphorylatedformoftheimmunizingpeptide.Specificconditionsforreactivityshouldbeoptimizedbytheenduser.Useformalin-fixedparaffin-embeddedsectionsforimmunohistochemistry.Nopre-treatmentofsampleisrequired.
Purity/Specifity
Anti-AKTpT308waspurifiedfromconcentratedtissueculturesupernatebyProteinAchromatography.ThisantibodyisspecificforhumanandmouseAKTproteinphosphorylatedatT308.ABLASTanalysiswasusedtosuggestcross-reactivitywithAKTpT308frommostvertebratespeciessourcesbasedon100%homologywiththeimmunizingsequence.Cross-reactivitywithAKTfromothersourceshasnotbeendetermined.Cross-reactivitywithAKT2andAKT3willlikelyoccur.Cancer,CellSignaling,Neuroscience,SignalTransductionresearch.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
品牌介绍
美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。
