Rockland/AKT1 Western化学发光印迹试剂盒/KCAI51/1试剂盒
商品编号：
KCAI51
品牌：
rockland-inc
市场价：
¥6180.00
美元价：
3708.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AKT1WesternChemiluminescentBlottingKitProperties Description AKT1WesternBlotKit-KCAI51 Host Mouse TargetSpecies Human KnownCrossReactivity human Clonality Monoclonal14E5.A2.B2.H9IgG2akappa Application WesternBlot:UserOptimized OtherDilution:UserOptimized ProductType Kit KitType ChemiluminescentWesternBlotKit PhysicalState n/a ShippingCondition DryIce Format IgG2a Label Unconjugated Preservative WashbuffersMUSTNOTcontainSODIUMAZIDEorotherinhibitorsofperoxidaseactivity! StABIlizer None GeneName AKT1 RelevantLinks UniProtKB-P31749 AKTChemiluminescentWBlotKitProtocol |
AKT1WesternChemiluminescentBlottingKitDescription
Background
AKT1WesternBlotingkitallowsforthedetectionofendogenousproteinlevelsofhumanAKT1presentincelllysatesprovidedbytheuser.Asapositivecontrol,thiskitincludesaMDA-MB468wholecelllysateproventocontainAKT1.AfterproteinseparationbySDS-PAGEandtransfer,themembraneisprobedwithRockland"soptimizedAnti-AKT1antibody.Detectionofthemembraneboundantibody-antigencomplexisachievedbytheadditionofasecondaryantibodyconjugatedtotheenzymehorserADIshperoxidase.Theenzymereactswithaspecializedformulationofluminol,anextremelysensitive,non-radioactivesubstratethatemitslightandallowsvisualizationusingX-rayfilmorotherimagingmethods,includinghighlysensitiveCCDcamerasandimagingsystems.AKT1WesternBlotingisidealforinvestigatorsinvolvedinCellSignaling,Cancer,NeuroscienceandSignalTransductionresearch.
Synonyms
AKT1westernblotting,AKT-1,PKBantibody,PKBgammaantibody,PKBGAMMAantibody,PRKBGantibody,ProteinkinaseAkt1antibody,ProteinkinaseBgammaantibody,RAC-gammaserine/threonine-proteinkinase,RAC-PK-gamma,ChemiluminescentWB,WesternBlottingKit,ChemiluminescentKit,PeroxidaseKit,ImmunoblottingKit
StorageCondition
Seekitinsertforcompleteinstructions.
ApplicationNote
UseRocklandImmunochemicals’Anti-AKT1ChemiluminescentKitforWesternBlottingfordetectionofhumanAKT1nativeandrecombinantproteinsbywesternblot.Expectabandapproximately56kDainsizecorrespondingtoAKT1protein.Thiskitisusefulforbothwesternblottinganddotblottingmethods.Pleasereadtheentireproductinsertpriortouse.
Purity/Specifity
RocklandImmunochemicals’ChemiluminescentWesternBlotKitforAKT1combinesallofthenecessaryreagentswitharapidprovenprotocolandtheextremelyhighsignaldetectionofourluminolchemiluminescentsubstrateforthedetectionofnativeand/orrecombinanthumanAKT1protein.Thekitisdesignedtodetectbothunphosphorylatedandphosphorylatedformsoftheprotein.CrossreactivitywithAKT1fromotherspecieshasnotbeendetermined,however,thesequenceoftheimmunogenshows100%identitytohuman,mouse,andrat,therefore,crossreactivityisexpected.Cross-reactivitywithAKT2andAKT3hasnotbeendetermined.Thiskitcontainssufficientsubstrateforupto5miniblotsat7.5x8cm2(1,800cm2)andisstableforatleast1yearwhenstoredasindicated.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
品牌介绍
美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。
