Rockland/碱性磷酸酶抗体/200-4134S/25µL
商品编号：
200-4134S
品牌：
rockland-inc
市场价：
¥1980.00
美元价：
1188.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
AlkalinePhosphataseAntibodyProperties Description Anti-ALKALINEPHOSPHATASE(E.coli)(RABBIT)Antibody-200-4134S Host Rabbit TargetSpecies Escherichiacoli KnownCrossReactivity E.coli Clonality Polyclonal Application PhysicalState Liquid(sterilefiltered) ShippingCondition DryIce Format IgG Label Unconjugated Concentration 1.0mg/mlbyUVabsorbanceat280nm Buffer 0.02MPotassiumPhosphate,0.15MSodiumChloride,pH7.2 Preservative 0.01%(w/v)SodiumAzide StABIlizer None GeneName phoA RelevantLinks UniProtKB-P00634 NCBI-P00634.1 GeneID-945041 |
AlkalinePhosphataseAntibodyDescription
Synonyms
rabbitanti-AlkalinePhosphataseAntibody,Akp2antibody,ALPantibody,Nagaoisozymeantibody,Placentalalkalinephosphataseantibody,Placentallikealkalinephosphataseantibody,PLAP1antibody,PLAPlikeantibody,Reganisozymeantibody,Kasaharaisozymeantibody
Immunogen
AlkalinePhosphatase[E.coli]
ImmunogenType
NativeProtein
StorageCondition
Storevialat-20°Corbelowpriortoopening.Thisvialcontainsarelativelylowvolumeofreagent(25µL).Tominimizelossofvolumedilute1:10byadding225µLofthebufferstatedabovedirectlytothevial.Recap,mixthoroughlyandbrieflycentrifugetocollectthevolumeatthebottomofthevial.Usethisintermediatedilutionwhencalculatingfinaldilutionsasrecommendedbelow.Storethevialat-20°Corbelowafterdilution.Avoidcyclesoffreezingandthawing.
ApplicationNote
ALKALINEPHOSPHATASEAntibodyhasbeenassayedagainst1.0ugofAlkalinePhosphatase[E.coli]inastandardELISAusingPeroxidaseconjugatedAffinityPurifiedanti-RabbitIgG[H&L](Goat)code#611-1302and(ABTS(2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid])code#ABTS-100asasubstratefor30minutesatroomtemperature. Aworkingdilutionof1:25,000to1:125,000ofthereconstitutionconcentrationissuggestedforthisproduct. A1:10,000dilutionhasbeenreportedtobeusefulforthedetectionbyimmunoblotofanintegralmembraneproteinfromagram-positiveorganismexpressedasaLacZ/PhoAchimericderivativeinE.coli. Theresearchershoulddetermineoptimaltitersforotherapplications.
Purity/Specifity
Anti-ALKALINEPHOSPHATASEisanIgGfractionantibodypurifiedfrommonospecificantiserumbyamulti-stepprocesswhichincludesdelipidation,saltfractionationandionexchangechromatographyfollowedbyextensivedialysisagainstthebufferstatedabove. Assaybyimmunoelectrophoresisresultedinasingleprecipitinarcagainstanti-RabbitSerumaswellaspurifiedandpartiallypurifiedAlkalinePhosphatase[E.coli]. CrossreactivityagainstAlkalinePhosphatasefromothertissuesandspeciesmayoccurbuthavenotbeenspecificallydetermined. SomeminimalcrossreactivityhasbeenreportedwhenlysatesofcellextractsoverproducingE.coliproteinsareprobed.
DisclaimerNote-General
Thisproductisforresearchuseonlyandisnotintendedfortherapeuticordiagnosticapplications.Pleasecontactatechnicalservicerepresentativeformoreinformation.AllproductsofanimaloriginmanufacturedbyRocklandImmunochemicalsarederivedfromstartingmaterialsofNorthAmericanorigin.CollectionwasperformedinUnitedStatesDepartmentofAgriculture(USDA)inspectedfacilitiesandallmaterialshavebeeninspectedandcertifiedtobefreeofdiseaseandsuitableforexportation.Allpropertieslistedaretypicalcharacteristicsandarenotspecifications.Allsuggestionsanddataareofferedingoodfaithbutwithoutguaranteeasconditionsandmethodsofuseofourproductsarebeyondourcontrol.Allclaimsmustbemadewithin30daysfollowingthedateofdelivery.TheProspectiveusermustdeterminethesuitabilityofourmaterialsbeforeadoptingthemonacommercialscale.SuggestedusesofourproductsarenotrecommendationstouseourproductsinviolationofanypatentorasalicenseunderanypatentofRocklandImmunochemicals,Inc.Ifyourequireacommerciallicensetousethismaterialanddonothaveone,thenreturnthismaterial,unopenedto:RocklandInc.,P.O.BOX5199,Limerick,Pennsylvania,USA.
品牌介绍
美国Rockland是一家老牌抗体原产企业，其位于美国费城郊区的农场保有近20种实验动物，全部按美国农业部实验动物规范饲养和操作，是近20年来美国少数几家成功的大型实验动物商业基地。其产品通过贴牌委托生产，国际代理商和直销等方式行销全球。其中最著名的是全线二抗产品，以全面，价格合理为特色。今年，Rockland也加大了在一抗方面的投资力度，新生物分子的抗体数量每年都大幅增长，而这又是以其强大的重组蛋白表达能力为基础的。美国Rockland公司为生命科学领域提供丰富的、高质量的抗体，包括载脂蛋白、细胞周期/凋亡、细胞因子、细胞信号蛋白、酶类、细胞外基质、转录因子(NFkB)和泛素（Ubiquitin）等识别抗体，应用范围包括Western blot、免疫组化、免疫荧光和ELISA等。 另外，Rockland也生产特别齐全的二抗，包括纯化、生物素、HRP/ALP、荧光标记二抗，应用十分广泛。Rockland还对部分设施进行了GMP改造，以吸引大型制药厂的委托订单。同时又以GMP标准为蓝图，提高其他产品的质量标准和管理水平。Rockland还不断与时俱进，探索其他扩展产品线的渠道。利用周边广布著名高校和研究机构的便利，Rockland加快和科研单位合作，从深度和广度两维加速开发新产品，正在从一个OEM老厂走到前台，缩短与直接客户的距离。
